This two-day course offers an introduction to the foundational principles and hands-on techniques necessary for upstream bioprocessing. Day one covers the fundamentals of upstream processing and bioreactor control, combining lectures with hands-on practical sessions including suspension cell culture scale-up and rocker bioreactor operation. Day two builds on these skills with sessions on single-use stirred tank bioreactors and cell therapies, paired with practical training in adherent cell culture and single-use technologies. The course provides a blend of theory and practical skills essential for initiating and scaling mammalian cell culture processes across both suspension and adherent